The scientific question that broadly drives my research interests is trying to understand how subtle chemical and physiological change in a single originating diploid cell can generate an incredibly complex multi-cellular functional embryo. I broadly want to take my postdoctoral research in the direction of understanding either the chemical, physiological, or mechanical forces that help drive early embryo development, more specifically how cells can communicate and differentiate themselves to eventually form an infinitely more complex early embryo. I also want to utilize microscopy and computer modeling to help explore these biological phenomena.
⊿ Current Research
Graduate Research with Dr. Paul Maddox (University of North Carolina, Chapel Hill, NC - Aug. 2013 – Present)
Primary Thesis Work:
|Comparing the unchanging stereotypical cell divisions of canonical centromeric workhorses (frog extract, yeast, and mammalian cells) to that of the highly dynamic and non-stereotypical cell divisions of an early embryo.|
Secondary (Undergrad-led) Work:
Project 1 (Neil Harwani, Parker McDuffie, Cole Barnhardt, & Ethan Hughes):
Characterizing known human regulators of CENP-A in C. elegans embryos via RNAi knockdowns.
Project 2 (Sanjana Rao):
Characterizing known human regulators of CENP-A in C. elegans embryos by quantifying fluorescently tagged regulators.
Project 3 (Erin Rogers):
Quantifying CENP-A dynamics in C. elegans embryos that are re-entering the cell cycle utilizing custom-designed microfluidic devices.
|Design of micro-fluidic device to image many immobilized L1 larvae for long periods of time in addition to being able to feed them.|
⊿ Previous Research Experience
Undergraduate Research with Dr. Barbara Wakimoto (University of Washington, Seattle, WA -Aug. 2010 - Jun. 2011)
Designed, performed, and found candidates in a large-scale screen for genes responsible for fertilization-defective Drosophila melanogaster males. Mentored fellow undergraduate student in fly genetics, breeding, and maintaining populations, compiling and maintaining detailed records on populations, dissecting adult flies, and phenotyping informative crosses.
Biological Science Technician (US Army Corps of Engineers, Seattle, WA - Jul. 2009 – Apr. 2011)
Collected water samples and biological specimens for analysis of baseline ecological, aesthetic, and physical environmental conditions. Compiled, organized, and utilized data in order to clearly and efficiently store and access collected information. This work performed contributed to the publication of a report by the US Army Corps of Engineers, which will be used in support of future legislation of funding.Gleason, Nancy C., Rhonda S. Lucas, and Scott V. Pozarycki. 2014. Fish, Bird, and Mammal Predator Populations and Consumption Rates of Juvenile Salmonids at Howard Hanson Dam, King County, Washington: Results for Baseline Year 2008. U.S. Army Corps of Engineers, Seattle District.